Scientist Solutions
Latest discussion from ScientistSolutions.com
Latest discussion from ScientistSolutions.com Life Science Discussion
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how remove anti-CD3 and anti-CD28 signaling
Hi;
I want to stimulation T cells for 18 hour with bound anti-CD3 and solution anti-CD28, then remove these signaling and culture cells for 3 day, how I should remove TCR signaling?
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mRNA Methylation Enriched in 3' UTRs & near Stop Codons
Get ready for epitranscriptomics:
bit.ly/Kp99bL (Biotechniques newsy intro)
bit.ly/Kp9XNL (Cell paper)
Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 32 UTRs and near Stop Codons
Meyer, Kate D.; Saletore, Yogesh; Zumbo, Paul; Elemento, Olivier; Mason, Christopher E.; Jaffrey, Samie R.
Cell doi:10.1016/j.cell.2012.05.003
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smears in Western blots? (are the proteins degraded) :((
I'm wondering how to properly store bacterial cell lysates? Do you add protease inhibitors before putting them at -20C or lower temperatures? When will the samples last?
Because, I've done Western blots of the cell lysates. The first time I did it, the bands looked neat and I still could quantify the bands. But when I tried to do the blots for the 3rd and 4th time, I only saw smears.. What happened???